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rapG [2019-06-19 11:38:33]
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rapG [2019-06-19 11:38:33]

response regulator aspartate phosphatase, controls [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity
locus
BSU40300
pI
4.81
mw
42.68 kDa
protein length
365 aa Sequence Blast
gene length
1098 bp Sequence Blast
function
control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity
product
response regulator aspartate phosphatase
essential
no
synonyms
yycM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.4|Protein phosphatases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.2|Response regulator aspartate phosphatase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.8|Quorum sensing]

    • Gene

    Coordinates
    4,140,260 → 4,141,357

    The protein

    Catalyzed reaction/ biological activity

  • control of [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] activity [Pubmed|12950930]

    • Protein family

  • RAP family (according to Swiss-Prot)

    • [SW|Domains]

  • six [SW|TPR repeat|tetratrichopeptide repeats] (according to UniProt)

    • Effectors of protein activity

  • binding of [protein|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|PhrG] to [protein|E1744329B6489F989F93F3E71E51E772E3926ABF|RapG] prevents it from binding [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU] [Pubmed|12950930]

    • Structure

  • [PDB|4I9E] ([protein|1F860F4E66A3D503D5A97F17D4AB0504BEFE7F9D|RapF], 26% identity) [pubmed|23526880]

    • Expression and Regulation

    Operons

    genes
    [gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]-[gene|3B4E60C698B8E4FA286E06B934EC0CEDD5F516A5|phrG]
    description
    [Pubmed|12950930]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16553878], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|12950930], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

    • regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [Pubmed|16430695], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR]: repression, [Pubmed|16553878], in [regulon|972401B6AF56EC67FE6F1C3FEA9E2A9FC77C7672|RghR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12850135], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • repressed by glucose (4.8-fold) ([protein|search|CcpA]) [Pubmed|12850135]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B821 (yycM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1820 NBRP B. subtilis, Japan]
  • BKE40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTAT
  • BKK40300 (Δ[gene|E1744329B6489F989F93F3E71E51E772E3926ABF|rapG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40300 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT, downstream forward: _UP4_ATTAAACGAACGGAGGTTAT

    • References

  • 16553878,16430695,12950930,23526880